Effect of Delonix Regia (Flame Tree) as Biorodenticide in Eliminating Wistar Albino Rats


  • Omaka Onyekachi Chief Research Consultant, Maeve academic Research Laboratory Awka, Anambra State, Nigeria
  • Nwajiobi Favour Okwukweka University Awka Anambra State, Nigeria




Comparative Study, Flame Tree, Biorodenticide, Eliminating, Wistar Albino


The study examined the effect of Delonix regia (flame tree) as biorodenticide in eliminating Wister albino rat. The plant samples used were collected from the Nnamdi Azikiwe university campus, dried for three days then the extract was extracted with the use of Soxhlet extractor using ethanol as extraction solvent after which the extract was concentrated in a rotary evaporator. The extract was administered to the wister Albino rate ate 200mg/ml dose and 400mg/ml dose. From the phytochemical analysis of the plant, the result showed that the plant contains phytochemicals such as steroids, alkaloid among others. The result also showed that the weight of the rat increased to 128.33±7.53b when given treatment as compared to their body weight(114.66±8.08a) before treatment. The result further indicated that at 200mg/ml, the weight of the rat is statistically significant than that at 400mg/ml.  From the analysis, it was noted that the organ weight of the rat and PCV of 200mg/ml dosage is higher (43.66±4.48b) than that 400mg/ml (30.33±2.603a) though this result is not statistically significant at P>0.05%.   Comparing the relative organ weight of the rat at 200mg/ml and at 400mg/ml, the weight at 400mg/ml for liver(3.68%) and kidney(0.79%) is higher than 200mg/ml relative liver weight(3.55%) and kidney(0.65%).   The difference in the relative organ weight is also not statistically significant at P>0.05%. There were no toxicity effects of the extract observed on the rat neither were there any mortality. Therefore, the study recommended that pharmaceutical industries should harness the potential of Delonix regia extract in the production of drugs and use it in the control of obesity among the human population and little dosage of the Delonix regia extract can be also be used in others to boast and maintain packed cell volume.


Arjariya A and Chaurasia K.(2009) Some medicinal plants among the tribes of Chhatarpur district (M. P) India. Ecoprint 16:43-50.

Bonnefoy, X., Kampen, H. and Sweeney, K. (2008). Public health significance of urban pests. WHO, Copenhagen: 1–56920) Buckle, A., Prescott, C., Ward, K. (1994). Resistance to the first and second generation anticoagulant rodenticides-a new perspective. In: Halverson WS, Crabb AC (eds) Proceedings of the 16th Vertebrate Pest Conference, Santa Clara. University of California, pp 138–141

Cameron, T. (1949) Diseases carried by house mice. Canadian Journal Camp Med Vet Sci 13:262

Dubey, N. K Shukla, R. Kumar, A. Singh, P. and. Prakash, B(2011) Global scenario on the application of natural products in integrated pest management programmes,” in Natural Products in Plant Pest Management, N. K. Dubey, Ed., CAB International. 1: 1–20.

Fowler DG.( 2006. ) Traditional fever remedies: a list of Zambian plants; p. 18.

Geduhn, A., Esther, A., Schenke, D., Mattes, H., Jacob, J. (2014). Spatial and temporal exposure patterns in non-target small mammals during brodifacoum rat control. Sci Total Environ 496:328–338

Hadler, M.R. and Buckle, A.P. (1992). Forty five years of anticoagulant rodenticides—past, present and future trends. In: Borrecco JE, Marsh RE (eds) Proceedings of the 15th Vertebrate Pest Conference, Newport Beach. University of California, pp 149–155

Halim, M..A.., Chowdhury, M..S.H.., Wadud, A.I., Uddin ,M.S., Sarker, S.K. and Uddin M.B. (2007). The use of plants in traditional health care practice of the Shaiji community in Southwestern Bangladesh. Journal of Trop Forest Science 19:168-75.

Kalandakanond- S. Thongsong, S. Daendee, B. Thongsong, and V. Chavananikul(2010), “The efficacy of pure natural repellents on rat responses using circular open field,” Thai Journal of Veterinary Medicine, 40(4): 411–418..

Khare C.P.(2007) (Ed.). Indian Medicinal Plants-An Illustrated Dictionary. Springer, New York, USA. p. 205-6.

Noumi E.and Dibakto T.W.(2000) Medicinal plants used for peptic ulcer in the Bangangte region, Western Cameroon. Fitoterapia 71

Ode, O.J., Saka, S. and Oladele G..M. (2011). The global relevance of traditional medicine and herbal plants, the nigerian perspective. International Journals of Applied Biol Pharm Technology 2:280-9.

Rahmatullah ,M., Haque, M.R.., Kamrul Islam, S., Jamal F., Anwarul Bashar A.B.M andAhmed,(2010). A Survey on the use of medicinal plants by folk medicinal practitioners in three areas of pirojpur district, bangladesh. American-Eurasian Journal Sustain Agric 4:247-59.

Rekha, D., Tamil, S.S., Bharathidasan, R., Panneerselvam, A., Ilakkiya, R. and Jayapal R. (2013). Study of medicinal plants used from koothanoallur and Marakkadai, Thiruvarur district of Tamil Nadu, India. Hygeia. Journal of Drugs Med 5:164-70.

Singla N. and Babbar B.K.(2010). Rodent damage and infestation in wheat and rice crop field: district wise analysis in Punjab State,” Indian Journal of Ecology, 37( 2): 184–188.

Singla, L. D., Singla, N., Parshad, V. R., Juyal, P. D. and Sood, K. (2008). “Rodents as reservoir of parasites in India,” Integrative Zoology, 3,(1): 21–26.

Singla, N and Parshad, V. R,(2007) “Antifeeding effects of a neem (Azadirachta indica)-based formulation botanical bird repellent against the house rat (Rattus rattus),” Indian Journal of Agricultural Sciences, 77,( 8): 502–504.

Singleton G. R. and Redhead, T. D.(1989). “House mouse plaguesin,” in Mediterranean Landscapes in Australia—Mallee Ecosystems and Their Management, J. C. Noble and R. A. Bradstock, Eds., CSIRO, Melbourne, Australia. pp. 418–433.

Singleton, G..R., Belmain, S, Brown, P.R and Hardy B (2010) Rodent outbreaks: ecology and impacts. International Rice Research Institute, Los Ban˜os Pp225-238

Singleton, G..R.., Brown, P.R., Jacob, J., Aplin, K. and Sudarmaji (2007). Unwanted and unintended effects of culling—a case for ecologically-based rodent management. Integrated Zoology 2:247–259.

Tripathi, P.. Dubey, N. K and. Shukla, A. K(2008) “Use of some essential oils as post-harvest botanical fungicides in the management of grey mould of grapes caused by Botrytis cinerea,” World Journal of Microbiology and Biotechnology, 24,(1): 39–46.

Zahin, M., Aqil, F., Khan, M.S.A and Ahmad I. (2010). Ethnomedicinal plants derived antibacterials and their prospects, in: Chattopadhyay D. (Ed.), Ethnomedicine: A Source of Complementary Therapeutics. Research Signpost, Kerala, India. p. 149-78.

Afolabi O. B. Nwantiti, A.O and Okafor, N. (2009). The chemical basis of the pathology of yam tuber. In: Advances in yam research: the biochemistry and technology of yam tuber (ed. Osuji, G.). Biochemical Soc. Of Nigeria in collaboration with Anambra State University of Technology, Nigeria pp.249-258.

Odugbemi, O.I(2016) Identification and Pathogenicity of Rot-Causing Fungal Pathogens Associated with Xanthosoma sagittifolium Spoilage in South Eastern Nigeria. International Journal of Agriculture Innovations and Research, 2 (6): 2319-1473.

GRIN,H(2017).). Effect of annealing and heat moisture conditioning on the physiochemical characteristics of Bambara groundnut Voandzeia substerran starch. Nahrung/Food, 46, 311 – 316.

Kim, C(2011). Mycological Deterioration and Pathogenicity Studies of Post-harvest Cassava. Food Science and Technology, 4 (2): 23-30.

Jayeola, D(2011)Fungi Associated with the Post-Harvest Loss of Sweet Potato. International Journal of Research Studies in Biosciences, 3 (9): 32-37

Anon,R( 2011) Fungi Associated with the storage Rot yam (Dioscorea rotundata Poir) in Yola, Adamawa state. Jour. of Sustainable Dev. In Agric. and Environ. 3(2):99-103.

Benjamin ,H,Asumugha, K. and Mbanaso, I. (2010), An assessment of the microbiological safety of dry yam processed in south West Nigeria, Africa Journal of Biotechnology, 6:157-161.

Sanibareet, E,Borate, R.N. and Nwakamma, P.T. (2009). Biodegradation of white yam (Dioscorea rotundata poir) and water yam (Dioscorea alata L.) slices dried under different conditions. KMITL Sci & Tech. Journal. 5(3):577-586.

Okoko, F(2009). Roles of Fungal Rots in Post-Harvest Storage Losses in some Nigerian varieties of Dioscorea species. British Microbiology Research Journal. 4 (3): 343-350.

El-Olemylet al,L,Chinen-chun, B.A. and Chukunda, F.A. (2010). Influence of some macro-elements, moisture and oxalic acid on rot of Cocoyam (Colocasia and Xanthosoma species). Int’l J. Crop Science 3(1): 10 – 14

Evans,E and Trease,B (2011). Assessment of Cassava Root and stem Rots in Ecozones of Togo and Evaluation of the Pathogen Virulence. Pak. Journal Bot. 42(3): 2059-2068.

Owoseni,M and Ogunnusi,G (2016)Cytogenetic techniques. In: Onyeike EN, Osuji JO (eds.) Res. Techniques in Biology. and Chem. Sci. Spring field Publishers Ltd., Owerri, Nig. pp. 70-83.

El-Olemyl, B.A; Adeniji, M.O; Zuofa, K. and Daniel-Kalio, L.A. (2010), Root and Corm rot of Cocoyams and Susceptibility of four cultivars of Cocoyams (Xanthosoma and Colocasia species) to microbial decay on Rivers State, Nigeria. African J. Root and Tuber Crops 3(1): 1–3

Okunjie,O,Esquenazi, R.C.I and Maduewesi, J.N.C (2017). Variability in pathogenicity of isolate and sensitivity to Benlate, Diathane M. 45. and PCNB of cocoyam rot fungi: Nig. J. of Plant Prot. 9: 74-81.

Ndukweet,R,Ewanishi, O. and Obetta, J. A.N. (2015). Fungi Associated with storage rots of cocoyams (Colocasia spp.) in Nsukka, Nigeria. Mycopathologia, 134: 21-25.


How to Cite

Omaka Onyekachi, & Nwajiobi Favour Okwukweka. (2021). Effect of Delonix Regia (Flame Tree) as Biorodenticide in Eliminating Wistar Albino Rats. International Journal of Agriculture, Biology & Environment (e-ISSN 2582-6107) DOI: 10.47504/IJAGRI, 2(1), 1–16. https://doi.org/10.47504/IJAGRI.2021.5137